适配体的解链温度和退火问题，求大神解惑？图二文字：MB-rp (1 m M), sDNA (1 m M), and OTA aptamer (APT) (1 m M) were annealed together at 90 ℃ for 5 min, followed by decreasing gradually to room temperature to form stable hybrid structures (sDNA/MB-rP/APT) as the ratiometric probe. The gold electrode (GE) was polished to obtain a mirror surface using 0.3 and 0.05 m m alumina powder in order and then the electrode was ultrasonic cleaned byethanol and deionized water, respectively. Afterthat, the electrode was electrochemically cleaned in H 2 SO 4 (0.5 M) by CV scanning in the potential range of 0.2e1.6 V until a stable voltam- metric peak obtained. Then drop sDNA/MB-rP/APT mix solution (10 m L) on the surface of electrode for 1 h to form the sDNA/MB-rP/ APT/GE through the AueS interaction, and then the modified electrode was immersed in MCH (1 mM) solution for 1h at room temperature to block the unoccupied sites.查看更多
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