对甘油三酯和脂肪酸GC-MS,可以用硫酸甲醇同时皂化和甲酯化吗?我看文献大部分是先用碱皂化,再 甲酯 化。 有几个文献直接用硫 酸甲醇 ,这样可以同时皂化和甲酯化吗? Extracted nonpolar metabolites were evaporated, saponified and esterified to form fatty acid methyl esters (FAMEs) through addition of 500 μl 2% (w/v) H2SO4 in methanol and incubation at 50 °C for 90–120 min.查看更多1个回答 . 3人已关注