采用软件进行蛋白消光系数的测定,一般是采用哪个值呢? 采用软件进行蛋白消光系数的测定,结果会给出两个值,一般是采用哪个值呢? Extinction coefficients:Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.Ext. coefficient 234400Abs 0.1% (=1 g/l) 1.610, assuming all pairs of Cys residues form cystinesExt. coefficient 232400Abs 0.1% (=1 g/l) 1.596, assuming all Cys residues are reduced是不是与蛋白序列中有没有游离的 半胱氨酸 有关? 求高手帮忙解答 查看更多1个回答 . 1人已关注