称了1mg的PD98059,溶解于水稀释的DMSO.吹打了一个小时,还是悬浮液!怎么办呢?其实我们实验室也是用这个抑制剂的,不过我们用的是细胞,具体的融解估计你也知道,我不太清楚是不是 和给动物用的一样,反正我们是直接 把 它按照自己需要的母液浓度直接用dmso稀释以后,分装保存. PD 098059 also acts as a specific inhibitor of the activation of MAPKK in Swiss 3T3 cells, suppressing by 80-90% its activation by a variety of agonists. PD 098059 (50 microM) inhibited the activation of p42MAPK and isoforms of MAP kinase-activated protein kinase-1 in Swiss 3T3 cells, but the extent of inhibition depended on how potently c-Raf and MAPKK were activated by any particular agonist and demonstrated the enormous amplification potential of this kinase cascade. 查看更多
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