求救MES缓冲溶液的配制?以配1升为例,称量9.76g MES、29.22g NaCl、5ml10%的Brij35,溶于1升蒸馏水,用3mol/L NaOH在pHS-3B酸度计上调节pH=6.0±0.1Mes buffer 配制1 MES buffer, 0.05M (pH = 6.0). Dissolve 9.76 g of MES (2-(N-morpholino) ethanesulphonic acid) in approximately 900 ml of deionised water. Adjust pH to 6.0 with 1 N NaOH. Transfer to a 1-litre volumetric flask and make to volume with deionised water. This solution may be kept for two weeks at room temperature。你可以配制成10X的,即0.5M,不需要高压灭菌2 mes缓冲液(0.04 M, pH 5.9)MES (2 ethanesulfonic acid) (Sigma M-8250) 1.56 g 以 KOH 調整 pH 至 5.9 後,加水至 200 mL 置於 4℃ 保存。MES缓冲液配制好后放置7-10天后再使用查看更多