弱弱的问一下？ novabiochem synthesis notes method 2-1: attachment of acids/phenols to merrifield resin & bromomethyl resins using cesium salts 1. dissolve acid/phenol in etoh (2ml/mmole) & add water (0.5ml/mmole). adjust ph to 7 with 2m aq. cs2co3. evaporate solution to dryness. add dioxane and evaporate to dryness. repeat evaporation with dioxane. 2. pre-swell merrifield resin in dcm for 1h & then wash with dmf. add cs salt(1.2eq.) in dmf to the resin and heat at 50degree over night. the reaction may be catalyzed by the addition of ki(0.1eq.). at the end of this time, wash the resin with 3*dmf, 3*dmf/water(1:1), 3*dmf, 3*dcm, 3*meoh. dry in vacuo over koh. the completeness of reaction can be checked by treating resin with a solution of 4-(4-nitrobenzyl)pyridine in dmf/dcm. a pink to violet color indicates the resence of unreacted halomethyl groups. 希望以上信息对楼主有用哦，[em07] novabiochem synthesis notes里有大量实用的多肽合成相关的实验操作，如果有人有需要，请发email至签名中的地址，只要在email中提供姓名、电话、邮编、地址、单位等信息，两周左右就可以得到免费的该资料，[em01]查看更多
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