请高手帮忙翻译“Sulfarsazen” 是什么? sulfarsazene 不知道中文名。 histochemical detection of zinc by means of sulfarsazene academician i. v. toroptsev* and v. a. e shchenko udc 616-008.924.7-074 methods of histochemieal detection of zinc by staining frozen and paraffin tissue sections with 0.02% solutions of sulfarsazene, made up in distilled water, alcohol, and a masking mlxture, have been developed. the location of zinc in the sections was shown by orange granules. the chief advantages of sulfarsazene over dithizone and other reagents are solubility in water and high selectivity with respect to zinc. existing methods of histochemical detection of zinc are based mainly on the use of dithizone [1, 4, 5, 7, 8]. although highly sensitive with respect to zinc, dithizone has very low selectivity: it can form complexes with 16 other heavy metals [6]. another disadvantage of dithizone, which considerably r e s t r i c t s its scope is its insolubility in water. for these reasons, attempts have been made to find other reagents for the histochemieal detection of zinc which would be free from these defects. the w r i t e r s ' attention was drawn to sulfarsazene, a compound synthesized by the all-union research institute of chemical reagents and specially pure chemical substances and recommended by them for the photometric and complexometrie determination of zinc [2, 3]. sulfarsazene (the monosodium salt of 4-nitrobenzene-l", 4-diazoamino-l,l'-azo-benzene-2" arseno- 4"-sulfonic acid) is a reddish-brown powder, soluble in water, slightly soluble in alcohols, and insoluble in nonpolar organic solvents. in 1 n naoh solution it gives a bluish-purple color. asosr b g~shi4oan6$asj~a mol. wt. =572,3z the color reaction of sulfarsazene with zinc is most selective in the presence of a mixture of tartaric and citric acids in ammonia buffer at ph 9.3-9.6. the sensitivity of the reaction is 0.2 pg zn in 5 ml solution. the determination of zn is not interfered with by equal quantities of cd, of 10 times the quantity of co, and of larger quantities of cu and other elements [2, 3]. experimental method when developing the methods, tissues with a known high zinc content were used: the pancreas and parotid gland of rabbits, the small intestine and prostrate gland of rats, the eye membranes of cats. the animals were sacrificed by decapitation, one part of each organ was taken for cutting from sections, and the other part was fixed in solutions. * academy of medical sciences of the ussr. department of pathological anatomy, tomsk medical查看更多
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