向NCBI上提交细菌序列后返回的邮件，我该怎么处理?BankIt2143545 : (1) [1] Are these sequences from: a) pure culture: a culture that contains only one microbial species or b) enrichment culture: use of selective culture media to enrich for a set of microorganisms with a particular phenotypic property, resulting in a partially purified, mixed culture. Please do not choose this option for purified strains. or c) bulk environmental DNA (uncultured): PCR-amplified directly from source/host DNA. If these are from bulk environmental DNA, were they amplified using: i) universal primers - amplifies DNA from a broad range of organisms or ii) species-specific primers - amplifies DNA from a single species [2] Provide unique names (such as clone, isolate, strain, or laboratory designation) that we can use to distinguish the separate sequence submissions. For a more detailed explanation, see the attachment. [3] Provide additional details describing the environmental conditions and geographic location where these sequences or organisms were isolated. If there are multiple conditions and/or locations, please provide the information as a spreadsheet or tab-delimited table: bankit no. sequenceID identifier environment ------------ ---------- ---------- ----------- bankit123456 Seq1 abc1 soil bankit123456 Seq2 def2 ocean water etc. [4] Complete feature annotation has not been included for some or all of the sequence(s) you have submitted. A. Please resubmit your sequence(s) with relevant features such as: - coding regions (CDS features), partial or complete, including nucleotide spans and reading frame. Using this information, our software will add the amino acid translations for you. - structural RNAs such as rRNAs, tRNAs, misc_RNAs, with nucleotide spans - features which may describe your sequence, such as repeat_regions, UTRs, promoters with nucleotide spans If we do not hear from you by Sep 5, 2018, all of your submission(s) will be deleted from the processing queue. We have appended instructions for adding features to records. We cannot accept annotation in text format, you must resubmit. If your annotated sequences have coding region features with internal stop codons or reading frameshifts, you will be asked to review and resubmit. Please be sure to analyze your data prior to resubmission. B. If you know the correct annotation, but are unsure how to add it, please contact GenBank Users Services at: info@ncbi.nlm.nih.gov and include this message with your request. C. If you are unable to determine the feature annotation, please provide a biological reason for the lack of annotation. Alternatively, you may choose to unverify your records and the following comment will be added: COMMENT GenBank staff is unable to verify sequence and/or annotation provided by the submitter. The commented record(s) will indicate that the data are unverified and your sequence(s) will not be included in NCBI BLAST databases. Send your reply to: gb-admin@ncbi.nlm.nih.gov and your resubmitted sequence(s) through BankIt For your reference, please find your preliminary flatfiles below with the information we currently have. Please reply using the current Subject line. Sincerely, Mark A. Landree, PhD Contractor GenBank Submissions Staff Bethesda, Maryland USA ******************************************************************* gb-admin@ncbi.nlm.nih.gov (for replies/updates to records in GenBank) info@ncbi.nlm.nih.gov (for general questions regarding GenBank) ******************************************************************* adding annotation using Bankit: [1] You must start a new BankIt submission; do not send an "update" to your submitted sequence. In addition, please include a note indicating this is a resubmission of bankit xxxxxxx or group number ######. [2] Features can be added in BankIt on the ''Features'' page by choosing either the ''Forms'' or the ''Files'' option. Choosing ''Files'' allows you to upload a 5-column feature table file, which is described at: https://www.ncbi.nlm.nih.gov/Sequin/table.html#Table Layout Choosing ''Forms'' allows you to input information for your choice of features from the following types: - Coding Region (CDS, with applicable Gene and/or mRNA) - RNA Features (tRNA, rRNA, etc) - Repeat Region (for simple repeats, mobile elements, satellites) - Other Features (5''UTR, 3''UTR, exons, introns, promoters, etc) Once you have chosen a specific feature, follow the instructions to input the features'' descriptive information (nucleotide intervals, protein name, rRNA name, gene name etc). [3] After reviewing your submission, click on finish submission to submit your record(s). 恳请大家帮帮忙，谢谢大家了。查看更多4个回答 . 9人已关注
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