马来酰亚胺与蛋白质中巯基反应问题? 用到一种偶联剂,用偶联剂中的 马来酰亚胺 与 蛋白质 中的巯基反应,但是偶联剂的使用说明上说“molecules to be reacted with the maleimide moiety must have free(reduced) sulfhydryls.For proteins,reduce disulfide bonds using 5mM TCPE(1:100 dilution of bond-breaker TCEP solution) for 30minutes at room temperature, followed by two passes through a suitable desalting column” 有哪位大侠知道这句话是什么意思啊 查看更多1个回答 . 17人已关注
求大神帮解决一个审稿人提的晶体问题,谢谢?求大神帮解决一个晶体学问题,非常感谢!数据见附件,结构完整 1) the absolute configuration of compound 1 is based on x-ray diffractometry but the flack parameter x(u) is too large in absolute value and uncertainty. the refinement should be repeated for the enantiomer. if x(u) remains too large, the ac cannot be assigned by this method.查看更多1个回答 . 12人已关注