适配体的解链温度和退火问题,求大神解惑?图一文字:Melting temperature (Tm) (sDNA to MB-rp)=67 ℃,Tm (sDNA to OTA-APT)= 79.7 ℃. Two Tm value were much higher than reaction temperature, which indicated the hybridization of the selected DNA are stable in the process of the OTA detection.查看更多