0.1%的DMSO加入淋巴细胞中共培养，会不会对淋巴细胞产生毒性呢? SP600125 (molecular weight = 220) is poorly soluble in water. Stock solutions of at least 20 mM can be made by using 100% dimethyl sulfoxide. As a general guide, for every 10 μM SP600125, it is recommended to include 0.1% DMSO in the culture media (e.g., 30 μM/0.3% DMSO). Prewarming of media and the use of serum protein may enhance solubility. SP600125 typically precipitates as fine needles that are visible at 50-fold magnification.The % of DMSO that is cytotoxic to cells is generally 0.1% and above. This does depend upon the cells you are using. ie macrophages more resistant than epithelial cell lines, primary cells are exquisitely sensitive to levels of DMSO Alternatively,you can browse the link below. 查看更多
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