小肽的质谱分析,真心求助?Fragments will only be detected if they carry at least one charge. If this charge is retained on the N terminal fragment, the ion is classed as either a, b or c. If the charge is retained on the C terminal, the ion type is either x, y or z. http://www.matrixscience.com/help/fragmentation_help.htmlSequence: EYSeq? ? #? ?? ? B? ?? ?? ?? ?Y? ?? ?? ?# (+1) ??E? ???1? ? 130.05046? ? 311.12436? ? 2 ??Y? ???2? ? 293.11379? ? 182.08176? ? 1 Sequence: YE Seq? ? #? ?? ? B? ?? ?? ?? ?Y? ?? ?? ?# (+1) ??Y? ???1? ? 164.07120? ? 311.12436? ? 2 ??E? ???2? ? 293.11379? ? 148.06103? ? 1 EY和YE的b,y离子不一样的。但是,实际情况很有可能是,因为碎裂的很多,还会出来很多其它的碎片峰,lz只能把所有比较显著的峰都给出合理的裂解途径了,才能证明它的连接就是你所希望的连接。。如果两种同分异构体混在一起了,那这就不是质谱能解决的范围了,至少要在LC能分开了才好定性,查看更多