是否有Rho-kinase（ROCK）特异性的激动剂呢?Lipid messengers such as arachidonic acid (AA) or sphingosine phosphorylcholine (SPC) are able to efficiently stimulates ROCK activity (5- to 6-fold increase) independently of RhoA"——Rho Kinases in Cardiovascular Physiology and Pathophysiology.Circulation Research. 2006;98:322 但可能不是特异性的。 查看更多
如何用核磁确定混合物中各组分的百分含量?1HNMR不但可以测定混合物中成分的相对含量,也可以通过加入合适的内标测定绝对含量,有很多文献.有个术语叫Quantitative NMR(QNMR).国外有1HNMR定量测定天然混合物的论文和综述文章.比如:Based on a brief revision of what constitutes state-of-the-art "quantitative experimental conditions" for 1 H quantitative NMR (qHNMR), this comprehensive review contains almost 200 references and covers the literature since 1982 with emphasis on natural products. It provides an overview of the background and applications of qHNMR in natural products research, new methods such as decoupling and hyphenation, and analytical potential and limitations, and compiles information on reference materials used for and studied by qHNMR. The dual status of natural products, being single chemical entities and valuable biologically active agents that need to be purified from complex matrixes, results in an increased analytical demand when testing their deviation from the singleton composition ideal. The outcome and versatility of reported applications lead to the conclusion that qHNMR is currently the principal analytical method to meet this demand. Considering both 1D and 2D 1 H NMR experiments, qHNMR has proved to be highly suitable for the simultaneous selective recognition and quantitative determination of metabolites in complex biological matrixes. This is manifested by the prior publication of over 80 reports on applications involving the quantitation of single natural products in plant extracts, dietary materials, and materials representing different metabolic stages of (micro)organisms. In summary, qHNMR has great potential as an analytical tool in both the discovery of new bioactive natural products and the field of metabolome analysis. 查看更多
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