请教汪老师？ 天然丰度的15nnmr是可以测定的,可以看下面的文章:文章中的化合物是 long-range 1 h- 15 n heteronuclear shift correlation at natural abundance: a tool to study benzothiazole biodegradation by two rhodococcus strains abstract the biodegradation of benzothiazole and 2-hydroxybenzothiazole by two strains of rhodococcus was monitored by reversed phase high-pressure liquid chromatography and by 1 h nuclear magnetic resonance (nmr). both xenobiotics were biotransformed into a hydroxylated derivative of 2-hydroxybenzothiazole by these two strains. the chemical structure of this metabolite was determined by a new nmr methodology: long-range 1 h- 15 n heteronuclear shift correlation without any previous 15 n enrichment of the compound. this powerful nmr tool allowed us to assign the metabolite structure to 2,6-dihydroxybenzothiazole. fig. 4. 1 h- 15 n ghmbc spectra of obt metabolite recorded in cd 3 od; the experiments were set up for assumed 3 j (delay = 80 ms) (a) or 4 j (delay = 140 ms) (b) long-range couplings.查看更多
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