小鼠大脑多脑区神经递质测定,标曲建在全脑匀浆中,基质效应是否有影响?RT我的课题是LC-MS法测定小鼠大脑多个脑区的某神经递质含量,标准曲线是将储备液配在全脑匀浆中处理得到的。因为实验中不可能测个海马就搞十几个海马来建标曲,太浪费老鼠了。现在审稿人提问,是否全脑中建立的标曲,其基质效应与某个特定脑区测定时的基质效应有差异?Individual sample sub-sections from different locations within the mouse brain were quantitated using a generic brain tissue method. Were any differences observed in terms of different matrix effects from different brain sub-sections ?谢谢大家!查看更多4个回答 . 8人已关注