药典杂质问题？Use a stainless steel column (20 cm × 4.6 mm) packed with end-capped octadecylsilyl silica gel for chromatography (5 µm) (Hypersil ODS is suitable). Use a flow rate of 2 mL per minute 48 volumes of methanol and 52 volumes of a phosphate buffer prepared by dissolving 4.91 g of sodium dihydrogen orthophosphate and 0.633 g of disodium hydrogen orthophosphate in sufficient water to produce 1000 mL. 完全按这个做的查看更多
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