如何利用pictar、miRanda、targetscan综合预测miRNA的靶基因?Dicer processing of the pre-miRNA is thought to be coupled with unwinding of the duplex. Generally, only one strand is incorporated into the miRISC, selected on the basis of its thermodynamic instability and weaker base-pairing relative to the other strand. The position of the stem-loop may also influence strand choice. The other strand, called the passenger strand due to its lower levels in the steady state, is denoted with an asterisk (*) and is normally degraded. In some cases, both strands of the duplex are viable and become functional miRNA that target different mRNA populations. 查看更多
用Rnase R消化RNA之后逆转成cDNA, 再跑qPCR时内参怎么选择?After treatment5% C. elegans total RNA was spiked-in followed by phenol-chloroform extraction of the RNA mixture. For controls the RNA was mock treated without the enzyme. 查看更多
第1天
关注盖德视界
添加小助手
