PSORT和TARGETP中的数据问题，mtp和SP，RC什么意思呢？Results of Subprograms *** Warning: 1st aa is not methyonine PSG: a new signal peptide prediction method N-region: length 8; pos.chg 2; neg.chg 1 H-region: length 4; peak value -3.50 PSG score: -7.90 GvH: von Heijne's method for signal seq. recognition GvH score (threshold: -2.1): -4.20 possible cleavage site: between 57 and 58 Seems to have no N-terminal signal peptide ALOM: Klein et al's method for TM region allocation Init position for calculation: 1 Tentative number of TMS(s) for the threshold 0.5: 1 Number of TMS(s) for threshold 0.5: 1 INTEGRAL Likelihood = -3.98 Transmembrane 22 - 38 PERIPHERAL Likelihood = 9.65 (at 59) ALOM score: -3.98 (number of TMSs: 1) MTOP: Prediction of membrane topology (Hartmann et al.) Center position for calculation: 29 Charge difference: -0.5 C( 0.0) - N( 0.5) N = C: N-terminal side will be inside membrane topology: type 2 (cytoplasmic tail 1 to 22) MITDISC: discrimination of mitochondrial targeting seq R content: 3 Hyd Moment(75): 6.31 Hyd Moment(95): 7.07 G content: 0 D/E content: 2 S/T content: 2 Score: -3.56 Gavel: prediction of cleavage sites for mitochondrial preseq R-2 motif at 16 PRT|EI NUCDISC: discrimination of nuclear localization signals pat4: none pat7: none bipartite: none content of basic residues: 11.4% NLS Score: -0.47 KDEL: ER retention motif in the C-terminus: none ER Membrane Retention Signals: XXRR-like motif in the N-terminus: WRAP none SKL: peroxisomal targeting signal in the C-terminus: none SKL2: 2nd peroxisomal targeting signal: none VAC: possible vacuolar targeting motif: none RNA-binding motif: none Actinin-type actin-binding motif: type 1: none type 2: none NMYR: N-myristoylation pattern : none Prenylation motif: none memYQRL: transport motif from cell surface to Golgi: none Tyrosines in the tail: none Dileucine motif in the tail: none checking 63 PROSITE DNA binding motifs: none checking 71 PROSITE ribosomal protein motifs: none checking 33 PROSITE prokaryotic DNA binding motifs: Sigma-54 interaction domain ATP-binding region A signature (PS00675): *** found *** LLLIGDSGVGKSCL at 34 NNCN: Reinhardt's method for Cytplasmic/Nuclear discrimination Prediction: cytoplasmic Reliability: 70.6 （所以这个蛋白质是作用于细胞质嘛） COIL: Lupas's algorithm to detect coiled-coil regions total: 0 residues查看更多
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