请问U2OS应该使用什么培养基?应用McCoy's 5a 以下是ATCC的培养条件：The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Corning? T-75 flasks (catalog #430641) are recommended for subculturing this product. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: 2 to 3 times per week 查看更多
毕赤酵母分泌表达上清过镍柱，表达上清需要如何处理才能上样?你的上清pH太低了，结合能力稍差的会直接不结合的，需要调节pH为弱碱性；当然酵母发酵发酵上清有一定的离子强度的，但为了尽量减少非特异吸附，通常Balance buffer为缓冲体系+300 or 500 mM NaCl，尽量调节发酵上清的离子轻度与其一致（不一致也可以，可能会影响最终的纯度及介质的载量）；Ni column的洗脱方式通常为Imidazole或pH查看更多
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