f[求助]求助反应机理？ < >大家好，现在有一个引文的反应例子，但是其中的反应机理我不知道自己理解的对不对，麻烦大家帮我分析一下，谢谢！Example 1 <BR><BR>5-(1-methylbutyl)-5-(.beta.-hydroxy-v-iodopropyl) Barbituric Acid <BR><BR>5 g (0.022 mol) of 5-(1-methylbutyl)-5-(allyl)-barbituric acid in 180 mL of water was heated under a reflux condenser to 75-85.degree. C. 6 g of iodide was added to the reaction mixture in small portions over a period of 2 hours. The reaction mixture was stirred and heated for 7 hours. The reaction mixture was cooled down and precipitated. Crystals were filtered off on a Buchner funnel and washed with water. The product was crystallized from ethanol (50 mL) to yield 5.57 g of the desired material. <BR><BR>5-(1-methylbutyl)-5-iodoacetonyl Barbituric Acid <BR><BR>1 g of 5-(1-methylbutyl)-5-(.beta.-hydroxy-v-iodopropyl) barbituric acid was dissolved in 75 mL of acetone. 30 mL of a 0.33 mol solution of potassium dichromate in 10% sulfuric acid was added. The reaction mixture was stirred for 2 hours at room temperature and subsequently extracted with 200 mL of ethyl acetate, washed with brine, water and the organic layer was dried over magnesium sulfate. Ethyl acetate was removed in vacuo and crude solid material crystallized from 70% ethanol. The yield was 519 mg of colorless crystals of the desired product. <BR><BR>Coupling of 5-(1-methyl)iodoacetonyl Barbituric Acid to Bovine Serum Albumin <BR><BR>226 mg of BSA was dissolved in 4.7 mL of phosphate (buffer 0.1 M, pH 8) and 520 ul of N,N'-dimethylformamide. To this solution was added 152 mg of 5-(1-methylbutyl)-5-iodoacetonyl barbituric acid in 530 uL of DMF. The reaction mixture was stirred at room temperature for 36 hours. The resulting solution was dialyzed exhaustively versus water and liophilized to give 208 mg of the desired conjugate. <BR></P>查看更多6个回答 . 1人已关注
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