明确文章中提到的有机试剂的名称信息?看到有一篇英文文章PCR-RFLP analyses of formalin-fixed fish eggs for the mapping of spawning areas in the Eastern Channel and Southern North Sea使用配置的 福尔马林 溶液能够保证后续组织 基因组 提取有较高的成功率,但是根据使用的有机试剂英文名称搜索不到确切的试剂名称或产品编号,由于有机化学知识匮乏,对于同样简称但具有不同构型的有机化学试剂无能为力,联系了一些试剂提供商也没有令人满意的答复,最初的引用原文也找不到,联系了原文作者也没有好的答复。因此只好在小木虫向各位化学专业同行请教。 文章中的原文描述如下:egg samples were fixed and conserved in formalin solution (Mastail and Battaglia, 1978; modified by Bigot, 1979). Briefly, a BHA solution was made up by adding 8 g BHA (buthylhydroxyanisol) to 500 mL monopropylen glycol and an EDTA solution was made by adding 20 g EDTA to 500 mL distilled water. Two litres of commercial formalin (36%) were buffered to pH 7 with sodium glycerophosphate and EDTA solution was added, while stirring. BHA solution and then 2 g ascorbic acid were added to distilled water up to 5 L to obtain a stock solution at 14.4% of formalin buffered at pH 7. The stock solution was agitated for about 20 min. Sea water was added to 2.4 mL of this solution to have a 40 mL of solution at 0.864% formalin in pill jars. This fixation solution allowed a better pigment conservation. After 2–3 weeks fixation, samples were transferred in storage solution without formalin (Steedman, 1976), containing 0.5 mL of propylene phenoxetol, 4.5 mL of propylene glycol and 95 mL of distilled water to limit DNA damaged due to formalin. 希望能够明确原文中提到的“monopropylen glycol”、“propylene phenoxetol“和”propylene glycol“的确切 中文名称 和CAS编号以方便试剂采购并避免实验多走弯路,在此感激不尽!查看更多1个回答 . 1人已关注