MicroRNA-221 inhibitor转染,但是老是转不进去?1. It seems that the green light in your images only showed a background level of fluorescence. What kind of dye did you lable your miRNA inhibitor? And how about the wavelength you used to detect the fluorescence ? 2. If the fluorescence was detected in the right way, and you followed the five steps' procedures provided by Invitrogen (http://tools.invitrogen.com/content/sfs/manuals/stealth_sirna_tsf_lf2k_man.pdf), I am afraid that your T24 cells were just not fit for the transfection by Lipo2000.You can try Lipofectamine RNAiMAX Reagent, which is developped for transfecting small RNAs, instead. I got greater than 90% transfection efficiencies when i transfected Hek293, Huh7 and HepG2 cells using RNAiMax. 查看更多