怎样才能使前体miRNA 主要表达 star miRNA (miRNA*)？My suggestion is using antisense inhibitor to block the dominant counter-mir from your pri-miRNA expression. But by doing this, I still don't think the mir* is "OVEREXPRESSED" (if this is what you want?), the mir*just becomes more dominant. The similar way is to change the C on the counter-mir to U (T) to eliminate its function but keep the base-pair. You can also try to make siRNA-like double-stranded RNA with GC base pairing in one end to favor the miRNA machinery to take the mir* sequence, however, DICER might chop your "siRNA" into 19-mer and I think that's the drawback. Alternatively, if you can change the base-pairing in the pre-miRNA to make mir* being favored, then it might work. 查看更多
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