有关二糖单体的分离提纯问题?各位朋友,近期在文献中看到有用高效 液相色谱 进行小分子分离提纯处理的,本人在实验室想尝试用普通过柱子进行类似小分子的分离,但是尝试了很多展开剂配比,仍然不能分开。 参考文献中的一段描述,但不明白如何进行。是否需要一套相关设备?恳请有相关经验的高人指点一二。谢谢啦 参开文献节选内容:“Shimadzu high performance liquid chromatography (HPLC )purification of the monomers were conducted on a Shimadzu HPLC system with a refractive index detector RID-10A and one Luna 5 μm C18(2) column with MeOH and H2O as an eluent. ESI-MS data were gathered on a Waters LCT premier with ACQUITY LC. ”(Biomacromolecules 2013, 14, 2561−2569; DOI: 10.1021/bm4003046)查看更多1个回答 . 10人已关注